Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-amino-1,3-propanediphosphonic acid | - |
Plasmodium falciparum | |
3-amino-3-(P-methylphosphinyl)propionic acid | - |
Plasmodium falciparum | |
3-amino-3-phosphonopropionic acid | - |
Plasmodium falciparum | |
3-amino-3-[P-(2-carboxypropyl)phosphinyl]propionic acid | - |
Plasmodium falciparum | |
4-amino-4-(P-phenylphosphinyl)butyric acid | 20% inhibition at 1.83 mM | Plasmodium falciparum | |
4-amino-4-phosphonobutyric acid | - |
Plasmodium falciparum | |
4-amino-4-[P-(2-carboxypropyl)phosphinyl]butyric acid | - |
Plasmodium falciparum | |
5-amino-5-phosphonopentanoic acid | - |
Plasmodium falciparum | |
EDTA | activity is reduced to 14% after incubating with 10 mM EDTA | Plasmodium falciparum | |
o-phenanthroline | activity is reduced to 8.3% after incubating with 20 mM o-phenanthroline | Plasmodium falciparum | |
Zn2+ | Zn2+ abolishes activity at 1 mM | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.135 | - |
Glu-7-amido-4-methylcoumarin | - |
Plasmodium falciparum | |
0.136 | - |
Glu-7-amido-4-methylcoumarin | in the presence of 1 mM Co2+ | Plasmodium falciparum | |
0.327 | - |
Asp-7-amido-4-methylcoumarin | in the presence of 1 mM Co2+ | Plasmodium falciparum | |
0.385 | - |
Asp-7-amido-4-methylcoumarin | - |
Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Plasmodium falciparum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enhanced activity | Plasmodium falciparum | |
additional information | activity is not enhanced by Ca2+, Fe2+, Mg2+, Mn2+, and Ni2+ | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
8 * 65000, SDS-PAGE | Plasmodium falciparum |
70000 | - |
8 * 70000, high performance liquid chromatography | Plasmodium falciparum |
520000 | - |
SDS-PAGE | Plasmodium falciparum |
560000 | - |
high performance liquid chromatography | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-agarose column chromatography and Superdex-200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Asp-7-amido-4-methylcoumarin + H2O | - |
Plasmodium falciparum | Asp + 7-amino-4-methylcoumarin | - |
? | |
Glu-7-amido-4-methylcoumarin + H2O | - |
Plasmodium falciparum | Glu + 7-amino-4-methylcoumarin | - |
? | |
additional information | no hydrolysis is observed against Leu-7-amido-4-methylcoumarin, Phe-7-amido-4-methylcoumarin, Ala-7-amido-4-methylcoumarin, Pro-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, Val-7-amido-4-methylcoumarin, Arg-7-amido-4-methylcoumarin, and Ile-7-amido-4-methylcoumarin | Plasmodium falciparum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 65000, SDS-PAGE | Plasmodium falciparum |
homooctamer | 8 * 70000, high performance liquid chromatography | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
M18 aspartyl aminopeptidase | - |
Plasmodium falciparum |
M18AAP | - |
Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable when stored for 1 h at 37°C over the pH range 6 to 11 | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
Glu-7-amido-4-methylcoumarin | at pH 7.5, in the absesence of Co2+ | Plasmodium falciparum | |
0.05 | - |
Asp-7-amido-4-methylcoumarin | at pH 7.5, in the absence of Co2+ | Plasmodium falciparum | |
0.33 | - |
Glu-7-amido-4-methylcoumarin | at pH 7.5, in the presence of 1 mM Co2+ | Plasmodium falciparum | |
0.354 | - |
Asp-7-amido-4-methylcoumarin | at pH 7.5, in the presence of 1 mM Co2+ | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Plasmodium falciparum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | stable when stored for 1 h at 37°C over the pH range 6 to 11 | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
4-amino-4-phosphonobutyric acid | at pH 7.5 | Plasmodium falciparum | |
0.0044 | - |
1-amino-1,3-propanediphosphonic acid | at pH 7.5 | Plasmodium falciparum | |
0.0271 | - |
3-amino-3-phosphonopropionic acid | at pH 7.5 | Plasmodium falciparum | |
0.0427 | - |
4-amino-4-[P-(2-carboxypropyl)phosphinyl]butyric acid | at pH 7.5 | Plasmodium falciparum | |
0.105 | - |
5-amino-5-phosphonopentanoic acid | at pH 7.5 | Plasmodium falciparum | |
0.183 | - |
3-amino-3-[P-(2-carboxypropyl)phosphinyl]propionic acid | at pH 7.5 | Plasmodium falciparum | |
0.395 | - |
3-amino-3-(P-methylphosphinyl)propionic acid | at pH 7.5 | Plasmodium falciparum |